Experimental  Liver  Peroxidation  Against  the  Background  of  Limb Ischemia -Reperfusion  Injury – Is  There  a  Pathogenic  Difference Between Its Modifications?

Nataliya Volotovska*

doi:10.26332/seemedj.v4i2.135

Nataliya Volotovska* Department of Physiology, Bioethics and Biosafety, I. Ya. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine http://orcid.org/0000-0003-4073-3148

DOI: https://doi.org/10.26332/seemedj.v4i2.135

Abstract

Introduction: Use of the haemostatic tourniquet is an important step in providing first aid in open bleeding injuries. Tourniquet pressure on the extremity triggers local lipid peroxidation. However, the systemic influence of tourniquet thereof has not been fully studied.

Aim: This study aimed to evaluate the changes that occur against the background of ischemia-reperfusion syndrome (IRS) and modifications of trauma in the main gland for detoxification – the liver.

Materials and Methods: In order to estimate the liver condition under the effects of a haemostatic tourniquet, animals were divided into 5 experimental groups, where modifications of hypoxia were performed as a result of bleeding, IRS and trauma due to mechanical fracture of the thigh.

Results: Biochemical study of the liver has shown that each type of such interventions caused the activation of lipid peroxidation in this organ. The highest increase of the malonic dialdehyde rate was observed in response to haemostatic tourniquet combined with blood loss. Additionally, its content was higher in the group combining mechanical trauma and the tourniquet compared to isolated trauma.

Conclusion: All types of our interventions caused hypoxia – as a result of isolated bleeding and cessation of blood flow due to the tourniquet. However, the release of overconcentration of toxic derivatives of rhabdomyolysis, which entered the blood stream after limb release, activated the pathological mechanisms of IRS, which included intensified lipid peroxidation in the liver.

(Volotovska N. Experimental Liver Peroxidation Against the Background of Limb Ischemia - Reperfusion Injury – Is There a Pathogenic Difference Between Its Modifications? SEEMEDJ 2020; 4(2); 1-11)